Protein Kinase C (PKC)-induced PKC Down-regulation

Protein Kinase C (PKC) and PKC Are the Major PKC Isotypes Involved in TCR Down-Regulation

Ahnak protein activates protein kinase C (PKC) through dissociation of the PKC-protein phosphatase 2A complex.

We have previously reported that central repeated units (CRUs) of Ahnak act as a scaffolding protein networking phospholipase Cgamma and protein kinase C (PKC). Here, we demonstrate that an Ahnak derivative consisting of four central repeated units binds and activates PKC-alpha in a phosphatidylserine/1,2-dioleoyl-sn-glycerol-independent manner. Moreover, NIH3T3 cells expressing the 4 CRUs of A...

A GFP-PKC fusion protein to investigate the mechanism of PKC down-regulation.

Since its cloning [4] Green Fluorescent Protein (GFP) from the bioluminescent jellyfish Aeqtroreu victoria has been established as a visual reporter of gene expression [5 ] . The red-shifted variant of GFP (RSGFP) used in ths work shows a sixfold brighter fluorescence, no hotoisomerization and much slower photobleaching than wt GFP. f he GFP coding sequence was removed from pRSGFP-C1 (Clontech)...

Nitric Oxide (NO) Induces Nitration of Protein Kinase C (PKC ), Facilitating PKC Translocation via Enhanced PKC -RACK2 Interactions

Activation of protein kinase C (PKC) by nitric oxide (NO) has been implicated in the development of cardioprotection. However, the cellular mechanisms underlying the activation of PKC by NO remain largely unknown. Nitration of protein tyrosine residues has been shown to alter functions of a variety of proteins, and NO-derived peroxynitrite is known as a strong nitrating agent. In this investiga...

Protein Kinase C (PKC)ζ Pseudosubstrate Inhibitor Peptide Promiscuously Binds PKC Family Isoforms and Disrupts Conventional PKC Targeting and Translocation.

PKMζ is generated via an alternative transcriptional start site in the atypical protein kinase C (PKC)ζ isoform, which removes N-terminal regulatory elements, including the inhibitory pseudosubstrate domain, consequently rendering the kinase constitutively active. Persistent PKMζ activity has been proposed as a molecular mechanism for the long-term maintenance of synaptic plasticity underlying ...